https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37115 R² = .0140; P = .0082; β = .1075), but not vitamin D₂ levels. It also correlated positively with female adult height (R² = .170; P = .0103; β = .1291) and negatively with the occurrence of female osteoporosis (P = .0495). All data were adjusted for age and gender as appropriate (unadjusted data also provided). From a contemporary perspective, vitamin D levels varied significantly according to season of blood sampling as might be predicted (P = .0009). Conclusions: Increased solar irradiance/UV exposure during the first trimester of pregnancy calibrates adult vitamin D metabolism, which is an important hormone in maintaining calcium balance. This may explain how very early lifecycle UV exposure can influence skeletal development (adult height) and modify risk for the skeletal degenerative disorder osteoporosis. The data demonstrate humans are tuned to the world (exposome) in ways we have not yet fully considered, and which are entrained at the earliest phase of the lifecycle.]]> Wed 19 Aug 2020 11:35:44 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38604 a production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-a production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-a production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-KB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-a (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-KB (~78%), IKB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.]]> Wed 17 Nov 2021 15:41:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43197 n = 649) Australian cross-sectional study population. Genetic analysis was used to score vitamin D- and folate-related gene polymorphisms (n = 22), along with two pigmentation gene variants (IRF4-rs12203592/HERC2-rs12913832). Red cell folate and vitamin D₃ were measured by immunoassay and HPLC, respectively. Results: i. Ultraviolet radiation (UVR) and pigmentation genes interact to modify blood vitamin levels; Light skin IRF4-TT genotype has greatest folate loss while light skin HERC2-GG genotype has greatest vitamin D₃ synthesis (reflected in both TOMS and seasonal data). ii. UV-wavelength exhibits a dose–response relationship in folate loss within light skin IRF4-TT genotype (305 > 310 > 324 > 380 nm). Significant vitamin D₃ photosynthesis only occurs within light skin HERC2-GG genotype, and is maximal at 305 nm. iii. Three dietary antioxidants (vitamins C, E, and β-carotene) interact with UVR and pigmentation genes preventing oxidative loss of labile reduced folate vitamers, with greatest benefit in light skin IRF4-TT subjects. The putative photosensitiser, riboflavin, did not sensitize red cell folate to UVR and actually afforded protection. iv. Four genes (5xSNPs) influenced blood vitamin levels when stratified by pigmentation genotype; MTHFR-rs1801133/rs1801131, TS-rs34489327, CYP24A-rs17216707, and VDR-ApaI-rs7975232. v. Lightest IRF4-TT/darkest HERC2-AA genotype combination (greatest folate loss/lowest vitamin D₃ synthesis) has 0% occurrence. The opposing, commonest (39%) compound genotype (darkest IRF4-CC/lightest HERC2-GG) permits least folate loss and greatest synthesis of vitamin D₃. Conclusion: New biophysical evidence supports the vitamin D-folate hypothesis for evolution of skin pigmentation.]]> Wed 14 Sep 2022 09:33:28 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51125 Tue 22 Aug 2023 15:51:11 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52824 Mon 30 Oct 2023 09:18:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37802 n = 619). Red blood cell folate, 25-hydroxyvitamin D (25(OH)D), and plasma Hcy levels were determined, and genotyping for 21 folate and vitamin D-related variants was performed. Erythemal dose rate accumulated over six-weeks (6W-EDR) and four-months (4M-EDR) prior to clinics were calculated as a measure of environmental UVR. Multivariate analyses found interactions between 6W-EDR and 25(OH)D levels (p_interaction = 0.002), and 4M-EDR and MTHFD1-rs2236225 (p_interaction = 0.006) in predicting Hcy levels. The association between 6W-EDR and Hcy levels was found only in subjects within lower 25(OH)D quartiles (<33.26 ng/mL), with the association between 4M-EDR and Hcy occurring only in subjects carrying the MTHFD1-rs2236225 variant. 4M-EDR, 6W-EDR, and MTHFD1-rs2236225 were also independent predictors of Hcy. Findings highlight nutrient-environment and gene-environment interactions that could influence the risk of Hcy-related outcomes.]]> Mon 26 Apr 2021 11:34:35 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42277  3R-TS. RCF was measured by chemiluminescent immunoassay and vitamin D2 and D3 by HPLC. Results: RCF and photosynthesized vitamin D3, but not RCF and dietary vitamin D2, exhibit a significant reciprocal association in spring and summer. Three folate genes (C677T-MTHFR, C1420T-SHMT, and 2R > 3R-TS) strengthen this effect in spring, and another (T401C-MTHFD) in summer. Effects are seasonal, and do not occur over the whole year. Conclusions: Findings are consistent with what might be required for the “folate-vitamin D-UV hypothesis of skin pigmentation” model. It suggests genetic influence in provision of one-carbon units by 5,10-methylene-H4folate, may be an important factor in what appears to be a clear seasonal relationship between vitamin D3 and folate status.]]> Fri 11 Aug 2023 09:43:44 AEST ]]>